Comparison of the composition and content of pulmonary surfactant among plateau zokors, plateau pikas and rats / 生理学报

In the present study, the composition and content of pulmonary surfactant (PS) were analyzed to explore the hypoxia adaptation mechanism in plateau zokors (Myospalax baileyi) and plateau pikas (Ochotona curzoniae). 36 plateau zokors and plateau pikas were trapped alive at the Laji Mountain in Guide County, Qinghai Province (at the altitude of about 3 600 m), and 36 Sprague-Dawley (SD) rats were purchased from the experimental animal center of Lanzhou University (at the altitude of about 1 500 m). All animals were lavaged after laboratory anesthesia, the blood in lung tissues was fully washed out and the lung tissues were then taken out to obtain the bronchoalveolar lavage fluid by bronchoalveolar lavage. The composition and content of phospholipids in the PS of three different kinds of animals were analyzed by using high performance liquid chromatography; the protein composition, content and types in the PS were analyzed by G-250 Coomassie brilliant blue method, polyacrylamide gel electrophoresis (PAGE) and mass spectrometry; the dissolved oxygen in the PS solutions were determined by using dissolved oxygen electrode. The results showed that the total contents of phospholipids in the PS were successively increased among plateau zokors, plateau pikas and SD rats (P 0.05). The relative content of PSe had no significant differences between plateau zokors and plateau pikas (P > 0.05), but both were significantly higher than that of SD rats (P < 0.01). The serum albumin (SA) was identified in the PS of three kinds of animals, including homologous tetramer protein containing heme, which is composed of hemoglobin β subunit, in plateau zokors and plateau pikas. Immunoglobulin (Ig) heavy chain was found in PS of plateau zokors and SD rats. The content of Ig heavy chain in plateau zokor was significantly higher than that in SD rats (P < 0.01), and the content of protein containing heme was significantly higher than that in plateau pikas (P < 0.05). The amount of dissolved oxygen was successively decreased in the PS among plateau zokors, plateau pikas and SD rats (P < 0.01), but it was significantly higher than that in saline (P < 0.01). These results suggest that the total content of proteins in the PS of plateau zokors and plateau pikas was significantly higher, while the total content of phospholipids was significantly decreased. There were high content of homologous tetramer protein containing heme in the PS of plateau zokors and plateau pikas. The relative content of DPPC, the main component of phospholipids, was significantly increased in plateau zokors. The changes of PS component and content improve the adaptability of the two plateau animals in hypoxia environment.

Status of AMH in Serum of Healthy Women in Shenzhen Area and Its Value in PCOS and POF Treatment / 现代检验医学杂志

Objective To investigate the current status of anti-mullerian hormone (AMH) in healthy women in the healthy childbearing age in Shenzhen area,and to explore its application value in polycystic ovary syndrome (PCOS) and prature ovarian syndrome (POF) treatment.Methods Collected 860 cases of medical health women of childbearing age in Shenzhen BaoanDistrict People's Hospital from January 2016 to March 2017 as control group,and at the same time selected 59 cases of patients with PCOS and 61 cases of patients with POF as observation group.Adopted DXI800 chemiluminescence analyzer to test serum AMH level of control group,patients with PCOS and POF group respectively before and 3 months after treatment,and the testing results were analyzed.Results The serum AMH level of Healthy women of childbearing age was 4.91 ±1.56 ng/ml,18～25 years old group was 5.38±1.27 ng/ml,31～35 and 36～42 years old group were 4.53±1.40 ng/ml and 3.95±1.16 ng/ml,being all lower than that in group 18～25 years old (t=3.082,5.066,all P＜0.05).While 26～30 years old group was 5.09± 1.45 ng/ml,and compared with 18～ 25,there was no statistically significant difference (t=0.892,P＞0.05).Before the treatment,the serum AMH level of PCOS was 10.13± 3.85 ng/ml,significantly higher than the control group,the difference was statistically significant (t=13.924,P＜0.01),while the serum AMH level of POF group and wait POF patients were 1.04±0.37 ng/ml and 2.39±0.87 ng/ml,significantly lower than the control group (t=10.913,8.042,all P＜0.05),and POF lower than wait POF group (t=2.875,P＜0.05).After treatment,the serum AMH level of PCOS was 5.22± 1.58 ng/ml,significantly lower than before treatment (t =11.106,P＜0.05),and there was no statistically significant difference between control group (t=1.036,P＞0.05),the serum AMH level of POF and wait POF were 4.49±1.32 ng/ml and 4.54± 1.47 ng/ml,significantly higher than the before treatment (t=9.608,7.253,all P＜0.05),and compared with the control group there were no statistically significant differences (t =1.209,0.918,all P＞0.05).Conclusion Before the treatment,the serum AMH levels of PCOS were increased significantly,and the serum AMH levels of POF and wait POF patients were significantly reduced,while the serum AMH levels of PCOS,POF and wait POF patients were all restored to normal levels after treatment.Therefore,AMH has certain application value in the diagnosis and treatment evaluation of women PCOS and POF disease.